Composite

Part:BBa_K4649009:Design

Designed by: Sophia Eowyn Hsu   Group: iGEM23_EastCoastBioCrew   (2023-09-11)


pstSCAB codon optimized for R. tropici


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2791
    Illegal XbaI site found at 3877
    Illegal SpeI site found at 3871
    Illegal PstI site found at 1667
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2791
    Illegal SpeI site found at 3871
    Illegal PstI site found at 1667
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2791
    Illegal BamHI site found at 1320
    Illegal BamHI site found at 3865
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2791
    Illegal XbaI site found at 3877
    Illegal SpeI site found at 3871
    Illegal PstI site found at 1667
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2791
    Illegal XbaI site found at 3877
    Illegal SpeI site found at 3871
    Illegal PstI site found at 1667
    Illegal NgoMIV site found at 207
    Illegal NgoMIV site found at 1248
    Illegal NgoMIV site found at 1430
    Illegal NgoMIV site found at 1490
    Illegal NgoMIV site found at 1988
    Illegal NgoMIV site found at 2823
    Illegal NgoMIV site found at 2912
    Illegal AgeI site found at 227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1850
    Illegal SapI site found at 1369


Design Notes

Codon optimized for Rhizobium tropici


Source

Escherichia coli strain K12 genome


References